ARG81862
Human CD320 ELISA Kit
Human CD320 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81862-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81862-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81862-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81862-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81862-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81862-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81862-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81862-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81862-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81862-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81862-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81862 Human CD320 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD320 in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD320 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5%; Inter-Assay CV: 6.3% |
| Alternate Names | TCBLR; CD320 antigen; CD antigen CD320; Transcobalamin receptor; 8D6 antigen; 8D6; 8D6A; TCblR; FDC-signaling molecule 8D6; FDC-SM-8D6 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD320 |
| Gene Full Name | CD320 molecule |
| Background | This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and is involved in B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011] |
| Function | Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81862 Human CD320 ELISA Kit standard curve image
ARG81862 Human CD320 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81862 Human CD320 ELISA Kit User manual |
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