ARG81883
Human CD32 ELISA Kit
Human CD32 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81883-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81883-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81883-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81883-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81883-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81883-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81883-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81883-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81883-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81883-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81883-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81883 Human CD32 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD32 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD32 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 23.45 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 46.9 - 3000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2%; Inter-Assay CV: 7.4% |
| Alternate Names | Fc-gamma-RIIc; Fc-gamma RII-c; CD32; CD antigen CD32; CD32B; IgG Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-c; FCG2; IGFR2; FcRII-c; FCGR2; CDw32 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P31994 Human Low affinity immunoglobulin gamma Fc region receptor II-b |
|---|---|
| Gene Symbol | FCGR2B |
| Gene Full Name | Fc fragment of IgG, low affinity IIb, receptor (CD32) |
| Background | The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Function | Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. [UniProt] |
| Highlight | Related products: CD32 antibodies; CD32 ELISA Kits; CD32 Duos / Panels; CD32 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated by the SRC-type Tyr-kinases LYN and BLK. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81883 Human CD32 ELISA Kit standard curve image
ARG81883 Human CD32 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81883 Human CD32 ELISA Kit User's manual |
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