ARG82759
Human CD305 / LAIR1 ELISA Kit
Human CD305 / LAIR1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82759-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82759-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82759-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82759-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82759-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82759-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82759-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82759-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82759-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82759-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82759-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82759 Human CD305 / LAIR1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD305 / LAIR1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CD305 / LAIR1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 50 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.5% Inter-Assay CV: 8.3% |
| Alternate Names | LAIR-1; hLAIR1; CD305; CD antigen CD305; Leukocyte-associated immunoglobulin-like receptor 1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6GTX8 Human Leukocyte-associated immunoglobulin-like receptor 1 |
|---|---|
| Gene Symbol | LAIR1 |
| Gene Full Name | leukocyte-associated immunoglobulin-like receptor 1 |
| Background | The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: CD305 antibodies; CD305 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK. N-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82759 Human CD305 / LAIR1 ELISA Kit standard curve image
ARG82759 Human CD305 / LAIR1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82759 Human CD305 / LAIR1 ELISA kit User manual |
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