ARG82452

Human CD300f ELISA Kit

Human CD300f ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82452-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82452-002 Standard 2 X 10 ng/vial 4°C
ARG82452-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82452-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82452-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82452-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82452-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82452-008 25X Wash buffer 20 ml 4°C
ARG82452-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82452-010 STOP solution 10 ml (Ready to use) 4°C
ARG82452-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82452 Human CD300f ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD300f in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CD300f
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%
Inter-Assay CV: 6.2%
Alternate Names Immune receptor expressed on myeloid cells 1; NK inhibitory receptor; NKIR; CD300 antigen-like family member F; IgSF13; CD antigen CD300f; IREM-1; IREM1; CLM1; CLM-1; CMRF35-like molecule 1; LMIR3; CD300f; Immunoglobulin superfamily member 13

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 146722 Human CD300LF

Swiss-port # Q8TDQ1 Human CMRF35-like molecule 1

Gene Symbol CD300LF
Gene Full Name CD300 molecule-like family member f
Background This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Function Acts as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Highlight Related products:
CD300 antibodies; CD300 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Phosphorylated on tyrosine. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG82452 Human CD300f ELISA Kit standard curve image

    ARG82452 Human CD300f ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82452 Human CD300f ELISA kit User manual download icon Download