ARG82450
Human CD300a ELISA Kit
Human CD300a ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82450-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82450-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82450-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82450-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82450-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82450-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82450-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82450-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82450-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82450-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82450-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82450 Human CD300a ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD300a in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CD300a |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4% Inter-Assay CV: 5.9% |
| Alternate Names | CMRF-35-H9; Inhibitory receptor protein 60; NK inhibitory receptor; CMRF35-H; CMRF35H9; CD antigen CD300a; IRC1/IRC2; IGSF12; CMRF35H; IRp60; IgSF12; CMRF35-like molecule 8; CMRF35-H9; CMRF-35H; CD300 antigen-like family member A; CLM-8; IRC1; IRC2; Immunoglobulin superfamily member 12 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD300A |
| Gene Full Name | CD300a molecule |
| Background | This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
| Function | Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: CD300 antibodies; CD300 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated on tyrosine. N-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82450 Human CD300a ELISA Kit standard curve image
ARG82450 Human CD300a ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82450 Human CD300a ELISA kit User manual |
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