ARG81922
Human CD28 ELISA Kit
Human CD28 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81922-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81922-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81922-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81922-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81922-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81922-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81922-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81922-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81922-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81922-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81922-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81922 Human CD28 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD28 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD28 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9%; Inter-Assay CV: 6.8% |
| Alternate Names | Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P10747 Human T-cell-specific surface glycoprotein CD28 |
|---|---|
| Gene Symbol | CD28 |
| Gene Full Name | CD28 molecule |
| Background | The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] |
| Function | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. [UniProt] |
| Highlight | Related products: CD28 antibodies; CD28 ELISA Kits; CD28 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | CD40LG induces tyrosine phosphorylation of isoform 3. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81922 Human CD28 ELISA Kit standard curve image
ARG81922 Human CD28 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81922 Human CD28 ELISA Kit User's manual |
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