ARG70302
Human CD276 / B7-H3 recombinant protein (ECD) (His-tagged, C-ter)
Human CD276 / B7-H3 recombinant protein (ECD) (His-tagged, C-ter) for Binding Activity,ELISA,SDS-PAGE and Human
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Human CD276 / B7-H3 recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | Binding, ELISA, SDS-PAGE |
| Target Name | CD276 / B7-H3 (ECD) |
| Species | Human |
| A.A. Sequence | Leu29 - Pro245 of Human CD276 / B7-H3 (NP_079516) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | B7-H3; B7 homolog 3; B7H3; 4Ig-B7-H3; B7RP-2; Costimulatory molecule; CD antigen CD276; CD276 antigen |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Human B7-H3 at 2 µg/ml (100 µl/well) can bind Anti-Human B7-H3 Antibody with a linear range of 1-4 ng/ml. |
|---|
Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CD276 |
|---|---|
| Gene Full Name | CD276 molecule |
| Background | The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Function | May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 57 kDa |
Images (2) Click the Picture to Zoom In
-
ARG70302 Human CD276 / B7-H3 recombinant protein (ECD) (His-tagged, C-ter) ELISA image
ELISA: The plate was coated with ARG70302 Human CD276 / B7-H3 recombinant protein (ECD) (His-tagged, C-ter) at 2 µg/ml (100 µl/well). Samples were detected with serially diluted anti-B7-H3 antibody.
-
ARG70302 Human CD276 / B7-H3 recombinant protein (ECD) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70302 Human CD276 / B7-H3 recombinant protein (ECD) (His-tagged, C-ter).
