ARG81792
Human CD271 ELISA Kit
Human CD271 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81792-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81792-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81792-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81792-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81792-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81792-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81792-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81792-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81792-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81792-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81792-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81792 Human CD271 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD271 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD271 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6%; Inter-Assay CV: 5.9% |
| Alternate Names | Gp80-LNGFR; CD271; CD antigen CD271; p75 ICD; Tumor necrosis factor receptor superfamily member 16; p75NTR; NGF receptor; TNFRSF16; p75(NTR); Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08138 Human Tumor necrosis factor receptor superfamily member 16 |
|---|---|
| Gene Symbol | NGFR |
| Gene Full Name | nerve growth factor receptor |
| Background | Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008] |
| Function | Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt] |
| Highlight | Related products: CD271 antibodies; CD271 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. Phosphorylated on serine residues. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81792 Human CD271 ELISA Kit standard curve image
ARG81792 Human CD271 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81792 Human CD271 ELISA Kit User's manual |
 Download
|
