ARG81437
Human CD26 ELISA Kit
Human CD26 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81437-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81437-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81437-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81437-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81437-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81437-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81437-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81437-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81437-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81437-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81437-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81437 Human CD26 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD26 in serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD26 / DPP4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 6.8% |
| Alternate Names | T-cell activation antigen CD26; ADCP2; ADCP-2; DPP IV; Adenosine deaminase complexing protein 2; CD26; EC 3.4.14.5; ADABP; Dipeptidyl peptidase IV soluble form; Dipeptidyl peptidase IV; Dipeptidyl peptidase 4; Dipeptidyl peptidase IV membrane form; TP103; DPPIV; CD antigen CD26 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DPP4 |
| Gene Full Name | dipeptidyl-peptidase 4 |
| Background | The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008] |
| Function | Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. [UniProt] |
| Highlight | Related products: CD26 antibodies; CD26 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing. N- and O-Glycosylated. Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81437 Human CD26 ELISA Kit standard curve image
ARG81437 Human CD26 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81437 Human CD26 ELISA Kit User's manual |
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