ARG80104
Human CD25 ELISA Kit
Human CD25 ELISA Kit for ELISA and Human
Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80104-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80104-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80104-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80104-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80104-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80104-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80104-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80104-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80104-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80104-010 | STOP solution | 12ml | 4°C |
| ARG80104-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80104 Human CD25 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD25 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with other soluble cytokine |
| Target Name | CD25 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 16 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01589 Human Interleukin-2 receptor subunit alpha |
|---|---|
| Gene Symbol | IL2RA |
| Gene Full Name | interleukin 2 receptor, alpha |
| Background | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009] |
| Function | Receptor for interleukin-2. [UniProt] |
| Highlight | Related products: CD25 antibodies; CD25 ELISA Kits; CD25 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
Images (1) Click the Picture to Zoom In
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ARG80104 Human CD25 ELISA Kit standard curve image
ARG80104 Human CD25 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80104 Human IL2sR alpha ELISA Kit User's manual |
 Download
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