ARG81779
Human CD244 ELISA Kit
Human CD244 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81779-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81779-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81779-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81779-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81779-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81779-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81779-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81779-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81779-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81779-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81779-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81779 Human CD244 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD244 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD244 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2%; Inter-Assay CV: 7.8% |
| Alternate Names | NK cell activation-inducing ligand; h2B4; Natural killer cell receptor 2B4; CD antigen CD244; 2B4; NAIL; NKR2B4; SLAM family member 4; Nmrk; SLAMF4; Signaling lymphocytic activation molecule 4; NK cell type I receptor protein 2B4 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD244 |
| Gene Full Name | CD244 molecule, natural killer cell receptor 2B4 |
| Background | This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009] |
| Function | Modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family. [UniProt] |
| Highlight | Related products: CD244 antibodies; CD244 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-linked glycosylation is essential for the binding to its ligand CD48. Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding. Phosphorylated by FYN and CSK on tyrosine residues following activation. Coligation with inhibitory receptors such as KIR2DL1 inhibits phosphorylation upon contact of NK cells with sensitive target cells. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81779 Human CD244 ELISA Kit standard curve image
ARG81779 Human CD244 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81779 Human CD244 ELISA Kit User's manual |
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