ARG82332
Human CD23 ELISA Kit
Human CD23 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82332-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82332-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82332-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82332-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82332-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82332-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82332-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82332-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82332-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82332-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82332-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82332 Human CD23 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD23 in serum, plasma (EDTA, heparin), cell culture supernatants and urine. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CD23 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin), cell culture supernatants and urine. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.1% Inter-Assay CV: 6.3% |
| Alternate Names | CD23A; BLAST-2; Lymphocyte IgE receptor; CLEC4J; Immunoglobulin E-binding factor; CD23; IGEBF; Fc-epsilon-RII; CD antigen CD23; FCE2; Low affinity immunoglobulin epsilon Fc receptor; C-type lectin domain family 4 member J |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P06734 Human Low affinity immunoglobulin epsilon Fc receptor |
|---|---|
| Gene Symbol | FCER2 |
| Gene Full Name | Fc fragment of IgE, low affinity II, receptor for (CD23) |
| Background | The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] |
| Function | Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Cell membrane; Lipid-anchor. Secreted. Note=Also exists as a soluble excreted form, sCD23. [UniProt] |
| Highlight | Related products: CD23 antibodies; CD23 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. The secreted form sCD23 is produced by ADAM10-mediated ectodomain shedding. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82332 Human CD23 ELISA Kit standard curve image
ARG82332 Human CD23 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82332 Human CD23 ELISA Kit User manual |
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