ARG81889
Human CD222 ELISA Kit
Human CD222 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81889-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81889-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81889-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81889-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81889-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81889-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81889-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81889-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81889-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81889-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81889-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81889 Human CD222 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD222 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD222 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.7%; Inter-Assay CV: 6.2% |
| Alternate Names | CD222; MPR 300; Insulin-like growth factor II receptor; M6P/IGF2R; MPRI; 300 kDa mannose 6-phosphate receptor; IGF-II receptor; CI Man-6-P receptor; M6P/IGF2 receptor; MPR1; CIMPR; Cation-independent mannose-6-phosphate receptor; CD antigen CD222; Insulin-like growth factor 2 receptor; M6P-R; CI-MPR; M6PR |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11717 Human Cation-independent mannose-6-phosphate receptor |
|---|---|
| Gene Symbol | IGF2R |
| Gene Full Name | insulin-like growth factor 2 receptor |
| Background | This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele. [provided by RefSeq, Apr 2013] |
| Function | Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. [UniProt] |
| Highlight | Related products: CD222 antibodies; CD222 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81889 Human CD222 ELISA Kit standard curve image
ARG81889 Human CD222 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81889 Human CD222 ELISA Kit User manual |
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