ARG81972
Human CD170 / Siglec 5 ELISA Kit
Human CD170 / Siglec 5 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81972-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81972-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81972-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81972-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81972-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81972-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81972-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81972-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81972-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81972-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81972-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81972 Human CD170 / Siglec 5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD170 / Siglec 5 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD170 / Siglec 5 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 23.45 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 46.9 - 3000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6%; Inter-Assay CV: 6.4% |
| Alternate Names | OBBP2; OB-binding protein 2; CD170; Sialic acid-binding Ig-like lectin 5; SIGLEC-5; CD antigen CD170; CD33 antigen-like 2; OB-BP2; CD33L2; Siglec-5; Obesity-binding protein 2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15389 Human Sialic acid-binding Ig-like lectin 5 |
|---|---|
| Gene Symbol | SIGLEC5 |
| Gene Full Name | sialic acid binding Ig-like lectin 5 |
| Background | This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012] |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. [UniProt] |
| Highlight | Related products: CD170 antibodies; CD170 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81972 Human CD170 / Siglec 5 ELISA Kit standard curve image
ARG81972 Human CD170 / Siglec 5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81972 Human CD170 / Siglec 5 ELISA Kit User's manual |
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