ARG81551
Human CD169 / Siglec 1 ELISA Kit
Human CD169 / Siglec 1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81551-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81551-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81551-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81551-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81551-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81551-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81551-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81551-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81551-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81551-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81551-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81551 Human CD169 / Siglec 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD169 / Siglec 1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD169 / Siglec 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.9% Inter-Assay CV: 7.9% |
| Alternate Names | CD169; Siglec-1; dJ1009E24.1; Sialic acid-binding Ig-like lectin 1; SIGLEC-1; CD antigen CD169; SN; Sialoadhesin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SIGLEC1 |
| Gene Full Name | sialic acid binding Ig-like lectin 1, sialoadhesin |
| Background | This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008] |
| Function | Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. [UniProt] |
| Highlight | Related products: CD169 antibodies; CD169 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81551 Human CD169 / Siglec 1 ELISA Kit standard curve image
ARG81551 Human CD169 / Siglec 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81551 Human CD169 / Siglec 1 ELISA Kit User's manual |
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