ARG81499

Human CD166 ELISA Kit

Human CD166 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81499-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81499-002 Standard 2 X 20 ng/vial 4°C
ARG81499-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81499-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81499-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81499-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81499-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81499-008 25X Wash buffer 20 ml 4°C
ARG81499-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81499-010 STOP solution 10 ml (Ready to use) 4°C
ARG81499-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81499 Human CD166 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD166 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD166
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.7%
Inter-Assay CV: 7.8%
Alternate Names MEMD; CD166 antigen; CD antigen CD166; Activated leukocyte cell adhesion molecule; CD166

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 214 Human ALCAM

Swiss-port # Q13740 Human CD166 antigen

Gene Symbol ALCAM
Gene Full Name activated leukocyte cell adhesion molecule
Background This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]
Function Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. [UniProt]
Highlight Related products:
CD166 antibodies; CD166 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Glycosylated. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81499 Human CD166 ELISA Kit standard curve image

    ARG81499 Human CD166 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81499 Human CD166 ELISA Kit User's manual download icon Download