ARG81499
Human CD166 ELISA Kit
Human CD166 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81499-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81499-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81499-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81499-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81499-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81499-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81499-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81499-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81499-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81499-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81499-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81499 Human CD166 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD166 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD166 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.7% Inter-Assay CV: 7.8% |
| Alternate Names | MEMD; CD166 antigen; CD antigen CD166; Activated leukocyte cell adhesion molecule; CD166 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ALCAM |
| Gene Full Name | activated leukocyte cell adhesion molecule |
| Background | This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011] |
| Function | Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. [UniProt] |
| Highlight | Related products: CD166 antibodies; CD166 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81499 Human CD166 ELISA Kit standard curve image
ARG81499 Human CD166 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81499 Human CD166 ELISA Kit User's manual |
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