ARG70470
Human CD153 / CD30 Ligand recombinant protein (Active) (His-tagged, C-ter)
Human CD153 / CD30 Ligand recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human TNFSF8 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CD153 / CD30 Ligand |
| Species | Human |
| A.A. Sequence | Gln63 - Asp234 |
| Expression System | E. coli |
| Protein Full Name | Tumor necrosis factor ligand superfamily member 8 |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-8 secretion in human PBMCs using a concentration range of 10 - 100 ng/mL. Note: Results may vary from different PBMC donors. |
| Alternate Names | TNFSF8; TNF Superfamily Member 8; CD30LG; CD153; Tumor Necrosis Factor (Ligand) Superfamily, Member 8; Tumor Necrosis Factor Ligand Superfamily Member 8; CD153 Antigen; CD30 Ligand; CD30-L; CD30L; Tumor Necrosis Factor Superfamily Member 8; Tumor Necrosis Factor Ligand 3A; CD30 Antigen Ligand; TNLG3A |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TNFSF8 |
|---|---|
| Gene Full Name | TNF Superfamily Member 8 |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene. |
| Function | Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. |
| Cellular Localization | Membrane |
| PTM | Disulfide bond, Glycoprotein |
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ARG70470 Human CD153 / CD30 Ligand recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70470 Human CD153 / CD30 Ligand recombinant protein (Active) (His-tagged, C-ter)
