ARG81996
Human CD146 ELISA Kit
Human CD146 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81996-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81996-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81996-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81996-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81996-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81996-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81996-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81996-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81996-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81996-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81996-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81996 Human CD146 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD146 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD146 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.9%; Inter-Assay CV: 6.2% |
| Alternate Names | Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; Cell surface glycoprotein MUC18; Melanoma cell adhesion molecule; CD146; MUC18; CD antigen CD146; S-endo 1 endothelial-associated antigen |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MCAM |
| Gene Full Name | melanoma cell adhesion molecule |
| Function | Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. [UniProt] |
| Highlight | Related products: CD146 antibodies; CD146 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81996 Human CD146 ELISA Kit standard curve image
ARG81996 Human CD146 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81996 Human CD146 ELISA Kit User's manual |
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