ARG83658
Human CD140b / PDGFRB ELISA Kit
Human CD140b / PDGFRB ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83658 Human CD140b / PDGFRB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD140b / PDGFRB in Serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CD140b / PDGFRB |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 100 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 μl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | PDGFRB; Platelet Derived Growth Factor Receptor Beta; PDGFR1; CD140b; JTK12; PDGFR; Platelet-Derived Growth Factor Receptor, Beta Polypeptide; Beta-Type Platelet-Derived Growth Factor Receptor; Platelet-Derived Growth Factor Receptor Beta; Platelet-Derived Growth Factor Receptor 1; CD140 Antigen-Like Family Member B; PDGF-R-Beta; EC 2.7.10.1; PDGFR-Beta; PDGFR-1; Beta Platelet-Derived Growth Factor Receptor; Activated Tyrosine Kinase PDGFRB; CD140b Antigen; NDEL1-PDGFRB; EC 2.7.10; IBGC4; PENTT; IMF1; KOGS |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P09619 Human Platelet-derived growth factor receptor beta |
|---|---|
| Gene Symbol | PDGFRB |
| Gene Full Name | Platelet Derived Growth Factor Receptor Beta |
| Background | The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Aug 2017] |
| Function | Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor. [Uniprot] |
| Cellular Localization | Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane. [Uniprot] |
| PTM | Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation. [Uniprot] |
Images (1) Click the Picture to Zoom In
-
ARG83658 Human CD140b / PDGFRB ELISA Kit standard curve image
ARG83658 Human CD140b / PDGFRB ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG83658 Human CD140b / PDGFRB ELISA Kit User manual |
 Download
|
