ARG81801

Human CD137 ELISA Kit

Human CD137 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81801-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81801-002 Standard 2 X 10 ng/vial 4°C
ARG81801-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81801-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81801-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81801-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81801-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81801-008 25X Wash buffer 20 ml 4°C
ARG81801-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81801-010 STOP solution 10 ml (Ready to use) 4°C
ARG81801-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81801 Human CD137 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD137 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD137
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.8%; Inter-Assay CV: 7.9%
Alternate Names ILA; CD137; T-cell antigen 4-1BB homolog; CDw137; T-cell antigen ILA; 4-1BB; CD antigen CD137; Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3604 Human TNFRSF9

Swiss-port # Q07011 Human Tumor necrosis factor receptor superfamily member 9

Gene Symbol TNFRSF9
Gene Full Name tumor necrosis factor receptor superfamily, member 9
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq, Jul 2008]
Function Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation. [UniProt]
Highlight Related products:
CD137 antibodies; CD137 ELISA Kits; CD137 recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (1) Click the Picture to Zoom In

  • ARG81801 Human CD137 ELISA Kit standard curve image

    ARG81801 Human CD137 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81801 Human CD137 ELISA Kit User's manual download icon Download