ARG81850
Human CD13 ELISA Kit
Human CD13 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81850-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81850-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81850-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81850-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81850-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81850-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81850-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81850-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81850-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81850-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81850-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81850 Human CD13 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD13 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD13 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6%; Inter-Assay CV: 6.6% |
| Alternate Names | AP-N; PEPN; LAP1; CD antigen CD13; Aminopeptidase M; gp150; Aminopeptidase N; EC 3.4.11.2; Myeloid plasma membrane glycoprotein CD13; APN; CD13; P150; AP-M; GP150; hAPN; Microsomal aminopeptidase; Alanyl aminopeptidase |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANPEP |
| Gene Full Name | alanyl (membrane) aminopeptidase |
| Background | Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008] |
| Function | Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. [UniProt] |
| Highlight | Related products: CD13 antibodies; CD13 ELISA Kits; CD13 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Sulfated. N- and O-glycosylated. May undergo proteolysis and give rise to a soluble form. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81850 Human CD13 ELISA Kit standard curve image
ARG81850 Human CD13 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81850 Human CD13 ELISA Kit User's manual |
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