ARG81850

Human CD13 ELISA Kit

Human CD13 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81850-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81850-002 Standard 2 X 20 ng/vial 4°C
ARG81850-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81850-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81850-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81850-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81850-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81850-008 25X Wash buffer 20 ml 4°C
ARG81850-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81850-010 STOP solution 10 ml (Ready to use) 4°C
ARG81850-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81850 Human CD13 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD13 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD13
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%; Inter-Assay CV: 6.6%
Alternate Names AP-N; PEPN; LAP1; CD antigen CD13; Aminopeptidase M; gp150; Aminopeptidase N; EC 3.4.11.2; Myeloid plasma membrane glycoprotein CD13; APN; CD13; P150; AP-M; GP150; hAPN; Microsomal aminopeptidase; Alanyl aminopeptidase

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 290 Human ANPEP

Swiss-port # P15144 Human Aminopeptidase N

Gene Symbol ANPEP
Gene Full Name alanyl (membrane) aminopeptidase
Background Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008]
Function Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. [UniProt]
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PTM Sulfated.

N- and O-glycosylated.

May undergo proteolysis and give rise to a soluble form. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81850 Human CD13 ELISA Kit standard curve image

    ARG81850 Human CD13 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81850 Human CD13 ELISA Kit User's manual download icon Download