ARG70252
Human CD123 / IL3RA recombinant protein (ECD) (His-tagged, C-ter)
Human CD123 / IL3RA recombinant protein (ECD) (His-tagged, C-ter) for Binding Activity,SDS-PAGE and Human
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Human CD123 / IL3RA recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | Binding, SDS-PAGE |
| Target Name | CD123 / IL3RA (ECD) |
| Species | Human |
| A.A. Sequence | Lys20 - Arg305 of Human CD123 / IL3RA (NP_002174.1) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | IL-3R subunit alpha; CD123; CD antigen CD123; IL3RAY; IL-3R-alpha; IL3RY; IL3RX; IL3R; IL-3RA; IL-3 receptor subunit alpha; hIL-3Ra; Interleukin-3 receptor subunit alpha |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant Human IL3 at 4µg/ml (100µl/well) can bind recombinant Human IL3RA, the EC50 of Human IL3RA is 5.3 µg/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >90% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL3RA |
|---|---|
| Gene Full Name | interleukin 3 receptor, alpha (low affinity) |
| Background | The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012] |
| Function | This is a receptor for interleukin-3. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 43 kDa |
Images (1) Click the Picture to Zoom In
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ARG70252 Human CD123 / IL3RA recombinant protein (ECD) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70252 Human CD123 / IL3RA recombinant protein (ECD) (His-tagged, C-ter).
