ARG70422
Human CCL4 / MIP1 beta recombinant protein (Active) (Tag Free)
Human CCL4 / MIP1 beta recombinant protein (Active) (Tag Free) for SDS-PAGE
Overview
| Product Description | E. coli expressed, Active Human CCL4 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CCL4 / MIP1 beta |
| Species | Human |
| A.A. Sequence | Ala24 - Asn92 |
| Expression System | E. coli |
| Protein Full Name | C-C motif chemokine 4 |
| Activity | Active |
| Activity Note | Determined by its ability to chemoattract BaF3 cells transfected with human CCR5. The ED50 for this effect is <10 ng/mL. |
| Alternate Names | CCL4; C-C Motif Chemokine Ligand 4; MIP-1-Beta; AT744.1; SCYA4; LAG1; Small Inducible Cytokine A4 (Homologous To Mouse Mip-1b); Macrophage Inflammatory Protein 1-Beta; Lymphocyte Activation Gene 1 Protein; G-26 T-Lymphocyte-Secreted Protein; Chemokine (C-C Motif) Ligand 4; T-Cell Activation Protein 2; C-C Motif Chemokine 4; MIP-1-Beta(1-69); SIS-Gamma |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | 50 mM Tris and 150 mM NaCl (pH 8.5) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CCL4 |
|---|---|
| Gene Full Name | C-C Motif Chemokine Ligand 4 |
| Background | The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. |
| Function | Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. |
| Cellular Localization | Secreted |
| PTM | Disulfide bond |
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ARG70422 Human CCL4 / MIP1 beta recombinant protein (Active) (Tag Free) SDS-PAGE image
SDS-PAGE analysis of ARG70422 Human CCL4 / MIP1 beta recombinant protein (Active) (Tag Free)
