ARG81659
Human CCL22 / MDC ELISA Kit
Human CCL22 / MDC ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81659-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81659-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81659-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81659-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81659-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81659-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81659-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81659-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81659-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81659-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81659-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81659 Human CCL22 / MDC ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CCL22 / MDC in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CCL22 / MDC |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2%; Inter-Assay CV: 7.5% |
| Alternate Names | CC chemokine STCP-1; Stimulated T-cell chemotactic protein 1; 3-69; Macrophage-derived chemokine; MDC; DC/B-CK; SCYA22; Small-inducible cytokine A22; ABCD-1; 7-69; 1-69; A-152E5.1; STCP-1; 5-69; C-C motif chemokine 22 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL22 |
| Gene Full Name | chemokine (C-C motif) ligand 22 |
| Background | This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. [provided by RefSeq, Sep 2014] |
| Function | May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active. [UniProt] |
| Highlight | Related products: CCL22 antibodies; CCL22 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81659 Human CCL22 / MDC ELISA Kit standard curve image
ARG81659 Human CCL22 / MDC ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81659 Human CCL22 / MDC ELISA Kit User's manual |
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