ARG81678
Human CCL21 / Exodus 2 ELISA Kit
Human CCL21 / Exodus 2 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81678-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81678-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81678-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81678-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81678-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81678-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81678-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81678-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81678-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81678-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81678-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81678 Human CCL21 / Exodus 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CCL21 / Exodus 2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CCL21 / Exodus 2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2%; Inter-Assay CV: 5.8% |
| Alternate Names | TCA4; Small-inducible cytokine A21; Beta-chemokine exodus-2; ECL; SCYA21; Secondary lymphoid-tissue chemokine; CKb9; 6Ckine; C-C motif chemokine 21; SLC |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL21 |
| Gene Full Name | chemokine (C-C motif) ligand 21 |
| Background | This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19). [provided by RefSeq, Sep 2014] |
| Function | Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. [UniProt] |
| Highlight | Related products: CCL21 antibodies; CCL21 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81678 Human CCL21 / Exodus2 ELISA Kit standard curve image
ARG81678 Human CCL21 / Exodus2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81678 Human CCL21 / Exodus 2 ELISA Kit User's manual |
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