ARG81506
Human CCL14 ELISA Kit
Human CCL14 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81506-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81506-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81506-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81506-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81506-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81506-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81506-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81506-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81506-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81506-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81506-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81506 Human CCL14 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CCL14 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CCL14 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6% Inter-Assay CV: 6.6% |
| Alternate Names | SY14; HCC-1; Chemokine CC-1/CC-3; HCC-3; SCYL2; CC-1; MCIF; CC-3; Small-inducible cytokine A14; HCC-1/HCC-3; 3-74; HCC-1(1-74); 4-74; 1-74; NCC-2; CKB1; 9-74; C-C motif chemokine 14; NCC2; SCYA14 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL14 |
| Gene Full Name | chemokine (C-C motif) ligand 14 |
| Background | This gene, chemokine (C-C motif) ligand 14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. [provided by RefSeq, Dec 2009] |
| Function | Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81506 Human CCL14 ELISA Kit standard curve image
ARG81506 Human CCL14 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81506 Human CCL14 ELISA Kit User's manual |
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