ARG82753
Human CANT1 ELISA Kit
Human CANT1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82753-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82753-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82753-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82753-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82753-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82753-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82753-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82753-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82753-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82753-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82753-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82753 Human CANT1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CANT1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CANT1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 6.3% |
| Alternate Names | Apyrase homolog; Soluble calcium-activated nucleotidase 1; EC 3.6.1.6; Putative MAPK-activating protein PM09; DBQD; SHAPY; Putative NF-kappa-B-activating protein 107; SCAN-1; SCAN1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8WVQ1 Human Soluble calcium-activated nucleotidase 1 |
|---|---|
| Gene Symbol | CANT1 |
| Gene Full Name | calcium activated nucleotidase 1 |
| Background | This protein encoded by this gene belongs to the apyrase family. It functions as a calcium-dependent nucleotidase with a preference for UDP. Mutations in this gene are associated with Desbuquois dysplasia with hand anomalies. Alternatively spliced transcript variants have been noted for this gene.[provided by RefSeq, Mar 2010] |
| Function | Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP. Involved in proteoglycan synthesis. [UniProt] |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane. Note=Processed form: Secreted. [UniProt] |
| Highlight | Related products: CANT1 antibodies; CANT1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82753 Human CANT1 ELISA Kit standard curve image
ARG82753 Human CANT1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82753 Human CANT1 ELISA kit User manual |
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