ARG82604

Human CALML5 ELISA Kit

Human CALML5 ELISA Kit for ELISA and Human

Overview

Product Description ARG82604 Human CALML5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CALML5 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity This kit could assay both natural and recombinant Human CALML5.

No significant cross-reactivity or interference was observed in the following samples:
Human: CALML3, GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL17A, IL18, IL21, IL22, IL23, MCP1, MIP1 beta, SCF, TGF beta 1, TNF alpha and VEGF.
Mouse: GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha.
Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha.
Target Name CALML5
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 23.5 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 46.9 - 3000 pg/ml
Sample Volume 20 µl
Precision Intra-Assay CV: 4.2%
Inter-Assay CV: 2.0%
Alternate Names CLSP; Calmodulin-like skin protein; Calmodulin-like protein 5

Application Instructions

Assay Time ~ 3.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51806 Human CALML5

Swiss-port # Q9NZT1 Human Calmodulin-like protein 5

Gene Symbol CALML5
Gene Full Name calmodulin-like 5
Background This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes. [provided by RefSeq, Jul 2008]
Function Binds calcium. May be involved in terminal differentiation of keratinocytes. [UniProt]
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  • ARG82604 Human CALML5 ELISA Kit standard curve image

    ARG82604 Human CALML5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

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