ARG82424
Human CA4 / Carbonic Anhydrase 4 ELISA Kit
Human CA4 / Carbonic Anhydrase 4 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82424-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82424-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82424-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82424-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82424-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82424-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82424-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82424-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82424-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82424-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82424-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82424 Human CA4 / Carbonic Anhydrase 4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CA4 / Carbonic Anhydrase 4 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CA4 / Carbonic Anhydrase 4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0% Inter-Assay CV: 6.0% |
| Alternate Names | CAIV; EC 4.2.1.1; Carbonic anhydrase IV; Car4; CA-IV; Carbonate dehydratase IV; RP17; Carbonic anhydrase 4 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CA4 |
| Gene Full Name | carbonic anhydrase IV |
| Background | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq, Jul 2008] |
| Function | Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| Highlight | Related products: Carbonic Anhydrase antibodies; Carbonic Anhydrase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82424 Human CA4 / Carbonic Anhydrase 4 ELISA Kit standard curve image
ARG82424 Human CA4 / Carbonic Anhydrase 4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82424 Human CA4 / Carbonic Anhydrase 4 ELISA Kit User manual |
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