ARG82856
Human CA3 / Carbonic Anhydrase 3 ELISA Kit
Human CA3 / Carbonic Anhydrase 3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82856-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82856-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG82856-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82856-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82856-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82856-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82856-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82856-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82856-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82856-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82856-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82856 Human CA3 / Carbonic Anhydrase 3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CA3 / Carbonic Anhydrase 3 in serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CA3 / Carbonic Anhydrase 3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.9% Inter-Assay CV: 5.5% |
| Alternate Names | CAIII; EC 4.2.1.1; Car3; CA-III; Carbonate dehydratase III; Carbonic anhydrase 3; Carbonic anhydrase III |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CA3 |
| Gene Full Name | carbonic anhydrase III |
| Background | Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008] |
| Function | Reversible hydration of carbon dioxide. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| PTM | S-glutathionylated in hepatocytes under oxidative stress. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82856 Human CA3 / Carbonic Anhydrase 3 ELISA Kit standard curve image
ARG82856 Human CA3 / Carbonic Anhydrase 3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82856 Human CA3 / Carbonic Anhydrase 3 ELISA Kit User manual |
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