ARG81593
Human Butyrylcholinesterase ELISA Kit
Human Butyrylcholinesterase ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81593-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81593-002 | Standard | 2 X 40 ng/vial | 4°C |
| ARG81593-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81593-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81593-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81593-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81593-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81593-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81593-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81593-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81593-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81593 Human Butyrylcholinesterase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Butyrylcholinesterase in serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Butyrylcholinesterase |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 312 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
| Standard Range | 625 - 40000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.6% Inter-Assay CV: 7.2% |
| Alternate Names | Butyrylcholine esterase; CHE2; CHE1; Choline esterase II; EC 3.1.1.8; Acylcholine acylhydrolase; E1; Cholinesterase; Pseudocholinesterase |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BCHE |
| Gene Full Name | butyrylcholinesterase |
| Background | Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq, Jul 2008] |
| Function | Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. [UniProt] |
| Highlight | Related products: Butyrylcholinesterase antibodies; Butyrylcholinesterase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. No other PTM detected (PubMed:20946535). The major N-glycan structures are of the complex diantennary type with 1 and 2 N-acetylneuraminic acid molecules (Neu5Ac) making up approximately 33% and 47% of the total N-glycans, respectively. Only low amounts of fucosylated diantennary N-glycans are detected (approximately 2%). Triantennary N-glycans with or without fucose amount to approximately 13%, whereas 5% of the total N-glycans are of the oligomannosidic or hybrid type. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81593 Human Butyrylcholinesterase ELISA Kit standard curve image
ARG81593 Human Butyrylcholinesterase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81593 Human Butyrylcholinesterase ELISA Kit User's manual |
 Download
|
