ARG82476
Human Brevican ELISA Kit
Human Brevican ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82476-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82476-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82476-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82476-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82476-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82476-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82476-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82476-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82476-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82476-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82476-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82476 Human Brevican ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Brevican in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Brevican |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.2% Inter-Assay CV: 7.0% |
| Alternate Names | BEHAB; Chondroitin sulfate proteoglycan 7; CSPG7; Brain-enriched hyaluronan-binding protein; Brevican core protein |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BCAN |
| Gene Full Name | brevican |
| Background | This gene encodes a member of the lectican family of chondroitin sulfate proteoglycans that is specifically expressed in the central nervous system. This protein is developmentally regulated and may function in the formation of the brain extracellular matrix. This protein is highly expressed in gliomas and may promote the growth and cell motility of brain tumor cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] |
| Function | May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans. [UniProt] |
| Cellular Localization | Isoform 1: Secreted, extracellular space, extracellular matrix. Isoform 2: Membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Contains mostly chondroitin sulfate (By similarity). O-glycosylated with a core 1 or possibly core 8 glycan. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82476 Human Brevican ELISA Kit standard curve image
ARG82476 Human Brevican ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82476 Human Brevican ELISA kit User manual |
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