ARG81562
Human Biglycan ELISA Kit
Human Biglycan ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81562-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81562-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81562-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81562-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81562-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81562-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81562-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81562-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81562-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81562-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81562-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81562 Human Biglycan ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Biglycan in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Biglycan |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.9% Inter-Assay CV: 5.5% |
| Alternate Names | Biglycan; Bone/cartilage proteoglycan I; SLRR1A; PGI; DSPG1; PG-S1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BGN |
| Gene Full Name | biglycan |
| Background | The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex. [provided by RefSeq, Nov 2009] |
| Function | May be involved in collagen fiber assembly. [UniProt] |
| Highlight | Related products: Biglycan antibodies; Biglycan ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81562 Human Biglycan ELISA Kit standard curve image
ARG81562 Human Biglycan ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81562 Human Biglycan ELISA Kit User's manual |
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