ARG82245
Human BNP ELISA Kit
Human BNP ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82245 Human BNP ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human BNP in serum and plasma. (Peptide extraction is recommended) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Cross-Reactivity: BNP-32 (Human): 100% BNP-32 (Rat): < 0.04% BNP-45 (Rat), BNP-26 (Pig), alpha ANP (1-28) (Human, Dog), Endothelin-1 (Human, Rat, Pig, Mouse, Dog, Bovine), Angiotensin II (Human) and VIP (Human, Rat, Pig): 0% |
| Target Name | BNP |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.26 ng/ml |
| Sample Type | Serum and plasma. (Peptide extraction is recommended) |
| Standard Range | 0.01 - 100 ng/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 15% |
| Alternate Names | 4-27; Natriuretic peptides B; 1-28; 1-29; 5-32; BNP; 1-32; 1-30; BNP-32; 5-29; 2-31; 4-30; 4-31; 4-32; 3-29; Gamma-brain natriuretic peptide; 3-30; 5-31; 3-32; 4-29 |
Application Instructions
| Assay Time | 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NPPB |
| Gene Full Name | natriuretic peptide B |
| Background | This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014] |
| Function | Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: BNP antibodies; BNP ELISA Kits; BNP Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The brain natriuretic peptide 32 form is cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82245 Human BNP ELISA Kit standard curve image
ARG82245 Human BNP ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82245 Human BNP ELISA Kit User manual |
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