ARG81404
Human BMP4 ELISA Kit
Human BMP4 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81404-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81404-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81404-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81404-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81404-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81404-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81404-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81404-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81404-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81404-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81404-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81404 Human BMP4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human BMP4 in cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Bov, Mk |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | BMP4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.4% Inter-Assay CV: 7.1% |
| Alternate Names | BMP2B; BMP-4; OFC11; Bone morphogenetic protein 2B; BMP2B1; Bone morphogenetic protein 4; MCOPS6; ZYME; BMP-2B |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BMP4 |
| Gene Full Name | bone morphogenetic protein 4 |
| Background | The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq, Jul 2008] |
| Function | Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity). [UniProt] |
| Highlight | Related products: BMP antibodies; BMP ELISA Kits; BMP recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81404 Human BMP4 ELISA Kit standard curve image
ARG81404 Human BMP4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81404 Human BMP4 ELISA Kit User's manual |
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