ARG70101
Human BMP15 recombinant protein (Active) (His-tagged, C-ter)
Human BMP15 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Premature ovarian failure. [UniProt]
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human BMP15 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | BMP15 |
| Species | Human |
| A.A. Sequence | Gln268 - Arg392 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is <17 ng/mL. |
| Alternate Names | BMP15; Bone Morphogenetic Protein 15; GDF9B; Growth/Differentiation Factor 9B; BMP-15; GDF-9B; ODG2; POF4 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | 20 mM sodium citrate and 0.2 M NaCl (pH 3.5) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | BMP15 |
|---|---|
| Gene Full Name | Bone Morphogenetic Protein 15 |
| Background | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate subunits of a disulfide-linked homodimer, or alternatively, a heterodimer, with the related protein, growth differentiation factor 9 (GDF9). This protein plays a role in oocyte maturation and follicular development, through activation of granulosa cells. Defects in this gene are the cause of ovarian dysgenesis and are associated with premature ovarian failure. [provided by RefSeq, Aug 2016] |
| Function | May be involved in follicular development. Oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Research Area | Premature ovarian failure. [UniProt] |
| PTM | Secreted. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG70101 Human BMP15 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70101 Human BMP15 recombinant protein (Active) (His-tagged, C-ter).
