ARG80154
Human BAFF ELISA Kit
Human BAFF ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80154-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80154-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80154-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80154-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80154-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80154-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80154-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80154-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80154-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80154-010 | STOP solution | 12ml | 4°C |
| ARG80154-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80154 Human BAFF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human BAFF in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human BAFF R, OX40 ligand, TWEAK, TRANCE, VEGI, GITR ligand, TNF-alpha |
| Target Name | BAFF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 30 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | BLYS; TALL1; Dendritic cell-derived TNF-like molecule; THANK; DTL; TNF- and APOL-related leukocyte expressed ligand 1; TNFSF20; CD257; BLyS; Tumor necrosis factor ligand superfamily member 13B; ZTNF4; CD antigen CD257; B lymphocyte stimulator; TALL-1; BAFF; B-cell-activating factor |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y275 Human Tumor necrosis factor ligand superfamily member 13B |
|---|---|
| Gene Symbol | TNFSF13B |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 13b |
| Background | BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. |
| Highlight | Related products: BAFF antibodies; BAFF ELISA Kits; BAFF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit |
| PTM | The soluble form derives from the membrane form by proteolytic processing. Isoform 2 is not efficiently shed from the membrane unlike isoform 1. N-glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG80154 Human BAFF ELISA Kit standard curve image
ARG80154 Human BAFF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80154 Human BAFF ELISA Kit User's manual |
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