ARG81827
Human Azurocidin ELISA Kit
Human Azurocidin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81827-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81827-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81827-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81827-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81827-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81827-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81827-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81827-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81827-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81827-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81827-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81827 Human Azurocidin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Azurocidin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Azurocidin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.5%; Inter-Assay CV: 7.0% |
| Alternate Names | Azurocidin; AZAMP; Heparin-binding protein; HBP; CAP37; HUMAZUR; NAZC; Cationic antimicrobial protein CAP37; AZU |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AZU1 |
| Gene Full Name | azurocidin 1 |
| Background | Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. The protein encoded by this gene is an azurophil granule antibiotic protein, with monocyte chemotactic and antimicrobial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, Dec 2014] |
| Function | This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Cleavage of the N-terminal propeptide which is composed of 7 amino acids occurs in two steps. The initial cleavage of 5 amino acids is followed by the cleavage of a dipeptide to produce the mature form. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81827 Human Azurocidin ELISA Kit standard curve image
ARG81827 Human Azurocidin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81827 Human Azurocidin ELISA Kit User's manual |
 Download
|
