ARG82745
Human Arylsulfatase B ELISA Kit
Human Arylsulfatase B ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82745-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82745-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82745-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82745-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82745-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82745-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82745-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82745-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82745-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82745-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82745-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82745 Human Arylsulfatase B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Arylsulfatase B in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Arylsulfatase B |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 32 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 5.8% |
| Alternate Names | N-acetylgalactosamine-4-sulfatase; EC 3.1.6.12; MPS6; ASB; G4S; Arylsulfatase B |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ARSB |
| Gene Full Name | arylsulfatase B |
| Background | Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Removes sulfate groups from chondroitin-4-sulfate (C4S) and regulates its degradation. Involved in the regulation of cell adhesion, cell migration and invasion in colonic epithelium. In the central nervous system, is a regulator of neurite outgrowth and neuronal plasticity, acting through the control of sulfate glycosaminoglycans and neurocan levels (By similarity). [UniProt] |
| Cellular Localization | Lysosome. Cell surface. [UniProt] |
| Highlight | Related products: Arylsulfatase antibodies; Arylsulfatase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82745 Human Arylsulfatase B ELISA Kit standard curve image
ARG82745 Human Arylsulfatase B ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82745 Human Arylsulfatase B ELISA kit User manual |
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