ARG82744
Human Arylsulfatase A ELISA Kit
Human Arylsulfatase A ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82744-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82744-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82744-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82744-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82744-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82744-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82744-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82744-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82744-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82744-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82744-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82744 Human Arylsulfatase A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Arylsulfatase A in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Arylsulfatase A |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.6% Inter-Assay CV: 5.9% |
| Alternate Names | ASA; Cerebroside-sulfatase; EC 3.1.6.8; Arylsulfatase A; MLD |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ARSA |
| Gene Full Name | arylsulfatase A |
| Background | The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2010] |
| Function | Hydrolyzes cerebroside sulfate. [UniProt] |
| Cellular Localization | Lysosome. [UniProt] |
| Highlight | Related products: Arylsulfatase antibodies; Arylsulfatase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82744 Human Arylsulfatase A ELISA Kit standard curve image
ARG82744 Human Arylsulfatase A ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82744 Human Arylsulfatase A ELISA kit User manual |
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