ARG80132
Human Apolipoprotein AI ELISA Kit
Human Apolipoprotein AI ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Neuroscience kit; Signaling Transduction kit
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Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80132-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80132-002 | Standard (Lyophilized) | 3 X 40 ng/vial | 4°C |
| ARG80132-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80132-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80132-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80132-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80132-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80132-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80132-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80132-010 | STOP solution | 12ml | 4°C |
| ARG80132-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80132 Human Apolipoprotein AI ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Apolipoprotein AI in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human ApoB, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoER2, ApoH, ApoJ, ApoM |
| Target Name | Apolipoprotein AI |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.6 ng/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 1.25 - 80 ng/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than9% |
| Alternate Names | ApoA-I; ProapoA-I; Apo-AI; Apolipoprotein A-I; Apolipoprotein A1; 1-242 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | APOA1 |
| Gene Full Name | apolipoprotein A-I |
| Background | This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008] |
| Function | Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. [UniProt] |
| Highlight | Related products: Apolipoprotein antibodies; Apolipoprotein ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Neuroscience kit; Signaling Transduction kit |
| PTM | Glycosylated. Palmitoylated. Phosphorylation sites are present in the extracellular medium. |
Images (1) Click the Picture to Zoom In
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ARG80132 Human Apolipoprotein AI ELISA Kit standard curve image
ARG80132 Human Apolipoprotein AI ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
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ELISA / Human
| Title | Download Link |
|---|---|
| ARG80132 Human Apolipoprotein AI ELISA Kit User's manual |
 Download
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