ARG81489
Human Angiopoietin like 4 ELISA Kit
Human Angiopoietin like 4 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81489-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81489-002 | Standard | 2 X 40 ng/vial | 4°C |
| ARG81489-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81489-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81489-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81489-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81489-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81489-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81489-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81489-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81489-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81489 Human Angiopoietin like 4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Angiopoietin like 4 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Angiopoietin like 4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 312.5 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 625 - 40000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6% Inter-Assay CV: 6.1% |
| Alternate Names | UNQ171; NL2; pp1158; FIAF; Angiopoietin-like protein 4; TGQTL; Hepatic fibrinogen/angiopoietin-related protein; Angiopoietin-related protein 4; ARP4; PGAR; HFARP; HARP |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANGPTL4 |
| Gene Full Name | angiopoietin-like 4 |
| Background | This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4. [provided by RefSeq, Sep 2013] |
| Function | Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation (By similarity). [UniProt] |
| Highlight | Related products: Angiopoietin antibodies; Angiopoietin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81489 Human Angiopoietin like 4 ELISA Kit standard curve image
ARG81489 Human Angiopoietin like 4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81489 Human Angiopoietin like 4 ELISA Kit User's manual |
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