ARG81402
Human Angiogenin ELISA Kit
Human Angiogenin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81402-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81402-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81402-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81402-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81402-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81402-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81402-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81402-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81402-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81402-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81402-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81402 Human Angiogenin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Angiogenin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Angiogenin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 39 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 78 - 5000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0% Inter-Assay CV: 6.6% |
| Alternate Names | RNASE5; RNASE4; ALS9; EC 3.1.27.-; RNase 5; Ribonuclease 5; Angiogenin; RAA1; HEL168 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANG |
| Gene Full Name | angiogenin, ribonuclease, RNase A family, 5 |
| Background | The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014] |
| Function | Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. [UniProt] |
| Highlight | Related products: Angiogenin antibodies; Angiogenin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81402 Human Angiogenin ELISA Kit standard curve image
ARG81402 Human Angiogenin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81402 Human Angiogenin ELISA Kit User's manual |
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