ARG80834
Human Androstenedione ELISA Kit
Human Androstenedione ELISA Kit for ELISA and Human
Signaling Transduction kit
Overview
| Product Description | ARG80834 Human Androstenedione ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Androstenedione in serum and plasma (EDTA). |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Androstenedione |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.021 ng/ml |
| Sample Type | Serum and plasma (EDTA). |
| Standard Range | 0.1 - 10 ng/ml |
| Sample Volume | 20 µl |
Application Instructions
| Assay Time | 1 h, 30 min (RT) |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Androstenedione |
|---|---|
| Background | The steroid hormone Androstenedione is one of the main androgens, besides Testosterone and Dehydroepiandrosterone. Testosterone, the most important biological active androgen, is derived from peripheral enzymatic conversion of Androstenedione. In males, androgens are secreted primarily by the Leydig cells of the testes, to some degree also in the adrenal cortex. In females, the androgens are secreted mainly in the adrenal glands and in the ovary. Around 10% of the androgens are derived from peripheral conversion, mainly of DHEA. Androstenedione and Testosterone show high diurnal variability. The highest levels are measured in the morning. At the age of puberty serum androstenedione levels rise, after menopause they decline again. High androstenedione levels are measured during pregnancy. In women, high levels of androstenedione (47-100% above normal) are generally found in hirsutism, mostly in combination with other androgens as testosterone and DHEA-S. Androstenedione overproduction is due to ovarian dysfunction or may be of adrenal origin. High circulating androstenedione levels are found in women with polycystic ovaries and 21-hydroxylase effect. Significant lower androstenedione levels are found in postmenopausal osteoporosis. |
| Highlight | Related products: Androstenedione ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Signaling Transduction kit |
Images (1) Click the Picture to Zoom In
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ARG80834 Human Androstenedione ELISA Kit example of standard curve image
ARG80834 Human Androstenedione ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG80834 Human Androstenedione ELISA Kit User's manual |
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