ARG81477
Human Aggrecan ELISA Kit
Human Aggrecan ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81477-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81477-002 | Standard | 2 X 100 ng/vial | 4°C |
| ARG81477-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81477-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81477-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81477-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81477-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81477-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81477-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81477-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81477-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81477 Human Aggrecan ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Aggrecan in serum, urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ACAN / Aggrecan |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.78 ng/ml |
| Sample Type | Serum, urine and cell culture supernatants. |
| Standard Range | 1.56 - 100 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8% Inter-Assay CV: 6.4% |
| Alternate Names | AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | ACAN |
|---|---|
| Gene Full Name | aggrecan |
| Background | This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008] |
| Function | This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. [UniProt] |
| Highlight | Related products: ACAN antibodies; ACAN ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81477 Human Aggrecan ELISA Kit standard curve image
ARG81477 Human Aggrecan ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81477 Human Aggrecan ELISA Kit User's manual |
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