ARG81587

Human Afamin / alpha Albumin ELISA Kit

Human Afamin / alpha Albumin ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81587-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81587-002 Standard 2 X 10 ng/vial 4°C
ARG81587-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81587-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81587-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81587-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81587-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81587-008 25X Wash buffer 20 ml 4°C
ARG81587-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81587-010 STOP solution 10 ml (Ready to use) 4°C
ARG81587-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81587 Human Afamin / alpha Albumin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Afamin / alpha Albumin in serum, urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Afamin / alpha Albumin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, urine and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%
Inter-Assay CV: 7.3%
Alternate Names Alpha-albumin; ALF; ALBA; ALB2; Afamin; Alpha-Alb

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 173 Human AFM

Swiss-port # P43652 Human Afamin

Gene Symbol AFM
Gene Full Name afamin
Background This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]
Function Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. [UniProt]
Highlight Related products:
Afamin antibodies; Afamin ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N-glycosylated; more than 90% of the glycans are sialylated. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81587 Human Afamin / alpha Albumin ELISA Kit standard curve image

    ARG81587 Human Afamin / alpha Albumin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81587 Human Afamin / alpha Albumin ELISA Kit User's manual download icon Download