ARG80131
Human Adiponectin ELISA Kit
Human Adiponectin ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Neuroscience kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80131-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80131-002 | Standard (Lyophilized) | 3 X 10 ng/vial | 4°C |
| ARG80131-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80131-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80131-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80131-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80131-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80131-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80131-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80131-010 | STOP solution | 12ml | 4°C |
| ARG80131-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80131 Human Adiponectin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Adiponectin in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Mouse adiponectin. |
| Target Name | Adiponectin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.08 ng/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 0.156 - 10 ng/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than 9.5% |
| Alternate Names | Adipose most abundant gene transcript 1 protein; ADPN; APM-1; Gelatin-binding protein; APM1; ACDC; Adiponectin; apM-1; ACRP30; ADIPQTL1; GBP28; 30 kDa adipocyte complement-related protein; Adipocyte, C1q and collagen domain-containing protein; Adipocyte complement-related 30 kDa protein |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ADIPOQ |
| Gene Full Name | adiponectin, C1Q and collagen domain containing |
| Background | This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010] |
| Function | Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. [UniProt] |
| Highlight | Related products: Adiponectin antibodies; Adiponectin ELISA Kits; Adiponectin Duos / Panels; Related news: A new role of fat – factory of circulating miRNAs New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Neuroscience kit; Signaling Transduction kit |
| PTM | Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes (By similarity). O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. |
Images (1) Click the Picture to Zoom In
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ARG80131 Human Adiponectin ELISA Kit standard curve image
ARG80131 Human Adiponectin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80131 Human Adiponectin ELISA Kit User's manual |
 Download
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