ARG81696
Human AXL ELISA Kit
Human AXL ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81696-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81696-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81696-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81696-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81696-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81696-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81696-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81696-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81696-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81696-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81696-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81696 Human AXL ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human AXL in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | AXL |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.4%; Inter-Assay CV: 8.3% |
| Alternate Names | UFO; Tyrosine-protein kinase receptor UFO; AXL oncogene; Tyro7; ARK; EC 2.7.10.1; JTK11 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P30530 Human Tyrosine-protein kinase receptor UFO |
|---|---|
| Gene Symbol | AXL |
| Gene Full Name | AXL receptor tyrosine kinase |
| Background | The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] |
| Function | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor. [UniProt] |
| Highlight | Related products: AXL antibodies; AXL ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion. Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81696 Human AXL ELISA Kit standard curve image
ARG81696 Human AXL ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81696 Human AXL ELISA Kit User's manual |
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