ARG82747
Human APLP1 ELISA Kit
Human APLP1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82747-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82747-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82747-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82747-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82747-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82747-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82747-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82747-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82747-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82747-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82747-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82747 Human APLP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human APLP1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | APLP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.3% Inter-Assay CV: 6.6% |
| Alternate Names | APLP; Amyloid-like protein 1; APLP-1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | APLP1 |
| Gene Full Name | amyloid beta (A4) precursor-like protein 1 |
| Background | This gene encodes a member of the highly conserved amyloid precursor protein gene family. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Function | May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. C30: Cytoplasm. Note=C-terminally processed in the Golgi complex. [UniProt] |
| Highlight | Related products: APLP1 antibodies; APLP1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Proteolytically cleaved by caspases during neuronal apoptosis. Cleaved, in vitro, at Asp-620 by caspase-3 (By similarity). N- and O-glycosylated. O-glycosylation with core 1 or possibly core 8 glycans. Glycosylation on Ser-227 is the preferred site to Thr-228. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82747 Human APLP1 ELISA Kit standard curve image
ARG82747 Human APLP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82747 Human APLP1 ELISA kit User manual |
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