ARG81991
Human AOC3 ELISA Kit
Human AOC3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81991-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81991-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG81991-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81991-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81991-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81991-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81991-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81991-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81991-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81991-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81991-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81991 Human AOC3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human AOC3 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | AOC3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8%; Inter-Assay CV: 6.6% |
| Alternate Names | HPAO; Semicarbazide-sensitive amine oxidase; VAP-1; Vascular adhesion protein 1; Copper amine oxidase; SSAO; Membrane primary amine oxidase; EC 1.4.3.21; VAP1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AOC3 |
| Gene Full Name | amine oxidase, copper containing 3 |
| Background | This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013] |
| Function | Cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has semicarbazide-sensitive (SSAO) monoamine oxidase activity. May play a role in adipogenesis. [UniProt] |
| Highlight | Related products: AOC3 antibodies; AOC3 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue. N- and O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81991 Human AOC3 ELISA Kit standard curve image
ARG81991 Human AOC3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81991 Human AOC3 ELISA Kit User's manual |
 Download
|
